ZFIN ID: ZDB-FIG-090826-19
Seipold et al., 2009 - Non-SMC condensin I complex proteins control chromosome segregation and survival of proliferating cells in the zebrafish neural retina. BMC Developmental Biology   9:40 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Day 5

Fig. 2 The zebrafish cap-g gene is mutated in s105. (A) Representation of the genetic map of the cap-g locus on linkage group 1 (LG1) and exon/intron structure of the cap-g transcript. Some of the markers utilized for cloning the mutation are indicated and the number of recombinants among 2982 meioses is indicated below each marker. (B) Schematic model of the associated condensin I complex. (C) Comparison of sequence data for wild-type and s105 mutant alleles. The s105 mutation generates a premature stop codon. (D) Schematic diagram of the Cap-G protein which contains several predicted HEAT domains. The s105 mutation generates a premature stop codon (red asterisk) that truncates more than half of the protein. (E) Comparison of cap-g expression with that of pcna by whole-mount in situ hybridization. Overlapping expression with pcna within the brain, the CMZ of the retina, which contains retinal stem cells, and within neuromasts of the lateral line organ (white asterisks) indicates that cap-g is required within proliferative cells.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ncapg WT standard conditions Prim-5 brain ISH
Protruding-mouth ciliary marginal zone ISH
Day 5 central nervous system ISH
Day 5 neuromast ISH
Day 5 retinal neural layer ISH
pcna WT standard conditions Prim-5 brain ISH
Protruding-mouth ciliary marginal zone ISH
Day 5 central nervous system ISH
Day 5 neuromast ISH
Day 5 retinal neural layer ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.