PUBLICATION
Non-SMC condensin I complex proteins control chromosome segregation and survival of proliferating cells in the zebrafish neural retina
- Authors
- Seipold, S., Priller, F.C., Goldsmith, P., Harris, W.A., Baier, H., and Abdelilah-Seyfried, S.
- ID
- ZDB-PUB-090716-9
- Date
- 2009
- Source
- BMC Developmental Biology 9: 40 (Journal)
- Registered Authors
- Abdelilah-Seyfried, Salim, Baier, Herwig, Harris, William A.
- Keywords
- none
- MeSH Terms
-
- Adenosine Triphosphatases/genetics
- Adenosine Triphosphatases/metabolism*
- Animals
- Apoptosis/genetics
- Apoptosis/physiology
- Cell Proliferation
- Chromosome Segregation/genetics
- Chromosome Segregation/physiology*
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/metabolism*
- Immunohistochemistry
- In Situ Hybridization
- Multiprotein Complexes/genetics
- Multiprotein Complexes/metabolism*
- Retina/cytology*
- Retina/metabolism*
- Tumor Suppressor Protein p53/metabolism
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 19586528 Full text @ BMC Dev. Biol.
Citation
Seipold, S., Priller, F.C., Goldsmith, P., Harris, W.A., Baier, H., and Abdelilah-Seyfried, S. (2009) Non-SMC condensin I complex proteins control chromosome segregation and survival of proliferating cells in the zebrafish neural retina. BMC Developmental Biology. 9:40.
Abstract
BACKGROUND: The condensation of chromosomes and correct sister chromatid segregation during cell division is an essential feature of all proliferative cells. Structural maintenance of chromosomes (SMC) and non-SMC proteins form the condensin I complex and regulate chromosome condensation and segregation during mitosis. However, due to the lack of appropriate mutants, the function of the condensin I complex during vertebrate development has not been described. RESULTS: Here, we report the positional cloning and detailed characterization of retinal phenotypes of a zebrafish mutation at the cap-g locus. High resolution live imaging reveals that the progression of mitosis between prometa- to telophase is delayed and that sister chromatid segregation is impaired upon loss of CAP-G. CAP-G associates with chromosomes between prometa- and telophase of the cell cycle. Loss of the interaction partners CAP-H and CAP-D2 causes cytoplasmic mislocalization of CAP-G throughout mitosis. DNA content analysis reveals increased genomic imbalances upon loss of non-SMC condensin I subunits. Within the retina, loss of condensin I function causes increased rates of apoptosis among cells within the proliferative ciliary marginal zone (CMZ) whereas postmitotic retinal cells are viable. Inhibition of p53-mediated apoptosis partially rescues cell numbers in cap-g mutant retinae and allows normal layering of retinal cell types without alleviating their aberrant nuclear sizes. CONCLUSIONS: Our findings indicate that the condensin I complex is particularly important within rapidly amplifying progenitor cell populations to ensure faithful chromosome segregation. In contrast, differentiation of postmitotic retinal cells is not impaired upon polyploidization.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping