FIGURE

Fig. 5

ID

ZDB-FIG-090817-13

Publication

McMahon et al., 2009 - Lmx1b is essential for survival of periocular mesenchymal cells and influences Fgf-mediated retinal patterning in zebrafish

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Fig. 5

Apoptosis and proliferation in lmx1b.1:GFP-positive cells following loss of Lmx1b function. (A) Immunoreactivity for activated Caspase-3 (red) and fluorescence for lmx1b.1:GFP^mw11 (green) in control MO or (B) lmx1bdMO ocular cryosections at 36 hpf. Arrows indicate cells positive for both activated Caspase-3 and lmx1b.1:GFP, while arrows indicate GFP-negative apoptotic cells. (C) Immunoreactivity for phosphoHistone (red) and fluorescence for lmx1b.1:GFP^mw11 (green) in control MO or (D) lmx1bdMO ocular cryosections at 36 hpf. Arrows indicate cells positive for both activated phosphoHistone-3 and lmx1b.1:GFP, while arrows indicate GFP-negative mitotic periocular cells. Dotted lines indicate position of the lens. Images shown are from representative sections (n = 24 total embryos for each condition from 2 experiments).

Expression Data

Gene:	GFP
Antibodies:	Ab4-casp3 Ab4-h3
Fish:	mw11Tg mw11Tg + MO2-lmx1bb + MO3-lmx1ba
Knockdown Reagents:	MO2-lmx1bb MO3-lmx1ba
Anatomical Term:	eye
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mw11Tg + MO2-lmx1bb + MO3-lmx1ba
Knockdown Reagents:	MO2-lmx1bb MO3-lmx1ba
Observed In:	apoptotic process
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 332(2), McMahon, C., Gestri, G., Wilson, S.W., and Link, B.A., Lmx1b is essential for survival of periocular mesenchymal cells and influences Fgf-mediated retinal patterning in zebrafish, 287-298, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.