FIGURE

Fig. 1

ID

ZDB-FIG-090717-61

Publication

Dong et al., 2009 - Heritable and lineage-specific gene knockdown in zebrafish embryo

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Fig. 1

Efficient inhibition of EGFP expression by zebrafish mir-30e precursor in vivo.

(A, B) Diagram of zebrafish miR-30e precursor sequence (A), and the EGFP sensor EGFP-2xPT^mir30e containing two tandem perfectly complementary target sites for miR-30e binding (2xPT) in its 3′UTR (B, upper). The capped EGFP sensor mRNAs were co-injected with either miR-30e or miR-155 precursor mRNAs into one-cell stage embryos (B, bottom). (C) A dramatic decrease of EGFP fluorescence in 24 hpf embryos co-injected with EGFP-2xPT^mir30e sensor (10 pg) and miR-30e precursor (50 pg). The DsRed mRNA was co-injected as an injection control (bottom panels). (D) Western blot analysis of 24 hpf embryos injected with EGFP-2xPT^mir30e sensor and miR-155 precursor or miR-30e precursor. The upper non-specific bands were shown as a loading control.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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