FIGURE

Fig. 6

ID

ZDB-FIG-090527-23

Publication

Phelps et al., 2009 - A two-step model for colon adenoma initiation and progression caused by APC loss

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Fig. 6

apc Control of Cellular Fate and Differentiation Are Mediated by ctbp1 and Are β-Catenin Independent

(A) Protein from 48 hpf apc^mcr zebrafish embryos injected with either 6 x His-APC^955–2075 or 6 x His-APC^955–2075-AALP were subjected to western blot analysis for β-catenin (top), ctbp1 (second), 6 x His (third), or vinculin (bottom).

(B) Embryos injected as above were fixed at 72 hpf and subjected to in situ hybridization for i-fabp.

(C) apc^mcr embryos were treated with DMSO or NS-398 or injected with ctbp1-directed morpholino in the presence or absence of NS-398. At 72 hpf, the embryos were fixed and subjected to in situ hybridization for i-fabp, NaPi, hoxa13a, or evx1.

All images are representative of at least three independent experiments.

Expression Data

Genes:	evx1 fabp2 hoxa13a
Fish:	WT apc^hu745/hu745 apc^hu745/hu745 + MO2-ctbp1
Knockdown Reagent:	MO2-ctbp1
Anatomical Terms:	cloaca intestine
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 137(4), Phelps, R.A., Chidester, S., Dehghanizadeh, S., Phelps, J., Sandoval, I.T., Rai, K., Broadbent, T., Sarkar, S., Burt, R.W., and Jones, D.A., A two-step model for colon adenoma initiation and progression caused by APC loss, 623-634, Copyright (2009) with permission from Elsevier. Full text @ Cell