ZFIN Figure: Phelps et al., 2009, Fig. 3

Fig. 3

KRAS and RAF1 Direct Stabilized β-Catenin to the Nucleus

(A) WT and KRAS-injected zebrafish embryos treated with PGE₂ were stained for DNA (magenta), PCNA (green), and β-catenin (green).

(B) Human 293 cells were transfected with constitutively active KRAS, RAF1, or MEK1 and either DMSO (top) or PGE₂ (bottom). Cells were stained for DNA (magenta) and β-catenin (green).

(C) 293 cells treated with DMSO, PGE₂ or PGE₂, and leptomycin B were stained for DNA (magenta) and β-catenin (green).

(D) 293 cells were transfected with control or KRAS- or RAF1-directed siRNA and treated with EGF and PGE₂ or vehicle and then stained for DNA (magenta) and β-catenin (green).

(E) 293 cells were subjected to an MTT assay (^*p < 0.05 versus DMSO; error bars, SEM).

Overlapping expression is shown in white. All images were captured using the same exposure and represent three independent experiments. Scale bar, 10 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 137(4), Phelps, R.A., Chidester, S., Dehghanizadeh, S., Phelps, J., Sandoval, I.T., Rai, K., Broadbent, T., Sarkar, S., Burt, R.W., and Jones, D.A., A two-step model for colon adenoma initiation and progression caused by APC loss, 623-634, Copyright (2009) with permission from Elsevier. Full text @ Cell