FIGURE

Fig. 6

ID

ZDB-FIG-090512-6

Publication

Covassin et al., 2009 - A genetic screen for vascular mutants in zebrafish reveals dynamic roles for Vegf/Plcg1 signaling during artery development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Artery differentiation defects in plcg1 mutants. (A–C) Classification schema to categorize level of artery marker gene expression in plcg1 mutant embryos. Differential interference contrast (DIC) images of zebrafish embryo trunks following whole mount in situ hybridization to detect ephrinb2a (efnb2a). (A) Normal ephrinb2a expression in the dorsal aorta (white arrow). (B) Weak ephrinb2a in distinct patches within the dorsal aorta (white arrows). (C) Loss of dorsal ephrinb2a expression classified as “absent”. (D) Quantification of artery marker gene expression in y13, y16, y18 and MPZy18 mutant embryos. (E, F) Transmitted light images of embryos at 30 hpf. Scale bar is 250 μM. (G, H) DIC images of embryos at 30 hpf. (E–H) Lateral views, anterior to the left, dorsal is up. (E, G) MPZplcg1^y18 mutant embryo. Scale bar is 30 μM; nt — neural tube, fp — floor plate, nc — notochord. Asterisked bracket denoted absence of defined dorsal aorta or posterior cardinal vein. F, H. Mplcg1^y18; Tg(fli1a:egfp)^y1 mutant embryo; DA — dorsal aorta, indicated by a bracket.

Expression Data

Genes:	dll4 efnb2a notch3
Fish:	WT plcg1^y18/y18 plcg1^y13/y13; y1Tg plcg1^y16/y16; y1Tg plcg1^y18/+; y1Tg/+ plcg1^y18/y18; y1Tg
Anatomical Term:	dorsal aorta
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	plcg1^y18/y18 plcg1^y18/+; y1Tg/+
Observed In:	dorsal aorta floor plate neural tube neural tube notochord posterior cardinal vein ventricular system
Stage:	Prim-15

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 329(2), Covassin, L.D., Siekmann, A.F., Kacergis, M.C., Laver, E., Moore, J.C., Villefranc, J.A., Weinstein, B.M., and Lawson, N.D., A genetic screen for vascular mutants in zebrafish reveals dynamic roles for Vegf/Plcg1 signaling during artery development, 212-226, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.