FIGURE

Fig. 3

ID

ZDB-FIG-090506-17

Publication

Rhinn et al., 2009 - Zebrafish gbx1 refines the midbrain-hindbrain boundary border and mediates the Wnt8 posteriorization signal

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Fig. 3

gbx1 acts as a mediator of the Wnt8 hindbrain posteriorizing signal. (A) Control embryo at 24 h, (B) gbx1-injected embryo (200 pg), (C) wnt8-injected embryo (400 pg). gbx1 overexpression mimics wnt8 gain-of-function; the embryos are truncated anteriorly. The red arrowhead indicates the position of the ear. (D-G′) Animal pole views at 60% epiboly after ISH with otx2. (D) Control embryo, (E, E′) gbx1-injected embryos (200 pg), (F) embryo injected with wnt8 morpholinos, (G, G′) embryos co-injected with gbx1 mRNA and wnt8 morpholinos. Expansion of the otx2 domain in the wnt8 morphant is rescued by co-injection with gbx1 mRNA. (H-K′) Dorsal views at the tailbud (tb) stage after ISH with otx2 (blue) and krox20 (red). (H) Control embryo, (I, I′) gbx1-injected embryos, (J) embryo injected with wnt8 morpholinos, (K, K′) embryos co-injected with gbx1 mRNA and wnt8 morpholinos. Loss of krox20 and posterior expansion of the otx2 domain in the wnt8 morphant is rescued by gbx1 mRNA.

Expression Data

Genes:	egr2b otx2b
Fish:	WT WT + MO1-wnt8a
Knockdown Reagent:	MO1-wnt8a
Anatomical Terms:	forebrain neural keel midbrain neural keel presumptive forebrain presumptive midbrain presumptive rhombomere 3 presumptive rhombomere 5
Stage Range:	Shield to Bud

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-wnt8a
Knockdown Reagent:	MO1-wnt8a
Observed In:	neural keel presumptive neural plate
Stage Range:	Shield to Bud

Phenotype Detail

Acknowledgments

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