FIGURE

Fig. 1

ID

ZDB-FIG-090506-15

Publication

Rhinn et al., 2009 - Zebrafish gbx1 refines the midbrain-hindbrain boundary border and mediates the Wnt8 posteriorization signal

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Overexpression of gbx1 induces posterior cell fate. (A) Wild-type (WT) embryo at 24 h and (B) after injection of 200 pg gbx1 mRNA; anterior brain structures are severely reduced. The ear is indicated by an arrowhead. (C, D) Staining of the forming axon tracts with an anti-acetylated tubulin antibody at 24 h; dorsal views, with anterior to the top. In the gbx1-injected embryo the hindbrain is severely enlarged compared to WT (arrowheads). (E, F) Duplications of ear structures are frequently observed (arrows). (G-I′) Series of embryosinjected with different doses of gbx1 mRNA (200 and 500 pg), analyzed at the tailbud stage after in situ hybridization with otx2 (blue) and krox20 (red). The otx2 domain progressively disappears and the krox20 domains shift to more anterior regions. (J) Dose-dependent gbx1 overexpression phenotypes. Higher concentrations (>500 pg) did not increase the observed phenotype. (A-D, K-M) Lateral views; (E-H, K′-M′) dorsal views.

Expression Data

Genes:	egr2b otx2b
Antibody:	Ab3-tubb
Fish:	WT
Anatomical Terms:	forebrain neural keel hindbrain axon Mauthner neuron midbrain neural keel presumptive rhombomere 3 presumptive rhombomere 5 trigeminal ganglion
Stage Range:	Bud to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neural Dev.