FIGURE

Fig. 1

ID

ZDB-FIG-090424-20

Publication

Paridaen et al., 2009 - Apc1-Mediated Antagonism of Wnt/beta-Catenin Signaling Is Required for Retino-Tectal Pathfinding in the Zebrafish

Other Figures

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Fig. 1

The apc^CA50a/CA50a mutation results in aberrant LEF/β-catenin transcription. (A, B) In wild-type embryos at 28 hpf (A), TGFP is absent from the retina. In apc mutants (B), TGFP is expressed in the retinal epithelium surrounding the lens (arrow). (C, D) At 36 hpf, TGFP is upregulated in the mutant lens and RGC layer (arrow in D). (E, F) Double in situ hybridization to apc1 and gap43, which marks differentiating neurons developing axons at 36 (E) and 48 hpf (F), shows that apc1 is expressed in areas of the retina that undergo differentiation. Dashed line indicates eye circumference (A– D) Lateral view, anterior to the left. (E, F) Ventral view, anterior to the left. (G, H) gap43 Expression in WT (G) and apc mutants (H) at 48 hpf shows disorganization of the GCL in the mutants. (I) apc1 Expression in the GCL and INL of the WT retina at 48 hpf. GCL, ganglion cell layer; INL, inner nuclear layer; le, lens; RGC, retinal ganglion cell. Scale bar = 50 μm.

Expression Data

Genes:	apc gap43 GFP
Fish:	WT apc^zf134/zf134 w25Tg apc^zf134/zf134; w25Tg
Anatomical Terms:	lens optic cup retinal ganglion cell layer retinal inner nuclear layer
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	apc^zf134/zf134 apc^zf134/zf134; w25Tg
Observed In:	canonical Wnt signaling pathway retina retinal ganglion cell layer
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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