FIGURE

Fig. S5

ID

ZDB-FIG-090408-60

Publication

Schebesta et al., 2009 - olig1 expression identifies developing oligodendrocytes in zebrafish and requires hedgehog and notch signaling

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Fig. S5

The olig1 morpholino blocks translation of eGFP in olig1:mem-eGFP transgenics while the olig2 morpholino interferes only with formation of eGFP+ oligodendrocytes. In uninjected control transgenic embryos (72 hpf), eGFP fluorescence is strongly observed in oligodendrocytes (red bracket) and in the cerebellum (red asterisks). In contrast, injection of 750 μM olig1-MO completely abolished translation of eGFP in transgenic embryos because the targeting sequence of olig1-MO is part of the transgenic construct. Injection of 250 μM olig2-MO blocks the development of oligodendrocytes, but does not block eGFP fluorescence in the cerebellum.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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