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Fig. S5 The olig1 morpholino blocks translation of eGFP in olig1:mem-eGFP transgenics while the olig2 morpholino interferes only with formation of eGFP+ oligodendrocytes. In uninjected control transgenic embryos (72 hpf), eGFP fluorescence is strongly observed in oligodendrocytes (red bracket) and in the cerebellum (red asterisks). In contrast, injection of 750 μM olig1-MO completely abolished translation of eGFP in transgenic embryos because the targeting sequence of olig1-MO is part of the transgenic construct. Injection of 250 μM olig2-MO blocks the development of oligodendrocytes, but does not block eGFP fluorescence in the cerebellum.