ZFIN ID: ZDB-FIG-090306-7
Sukumaran et al., 2009 - Early defects in photoreceptor outer segment morphogenesis in zebrafish ift57, ift88 and ift172 Intraflagellar Transport mutants. Vision Research   49(4):479-489 Full text @ Vision Res.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Term:
Stage: Pec-fin

Fig. 6 Western blot analysis of whole wild type and IFT mutant embryos at 60 hpf. Immunoreactivity against IFT52 protein (top row) was detected in wild type, ift57, ift88, and ift172 mutants. Immunoreactivity against IFT88 protein (middle row) was seen only in wild type, ift57 and ift172 mutants, thus showing that the ift88 mutant is null. Acetylated tubulin (AT) served as a control (bottom row).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ift52 Ab1-ift52 WT standard conditions Pec-fin whole organism WB
Ab1-ift52 ift172hi2211Tg/hi2211Tg standard conditions Pec-fin whole organism WB
Ab1-ift52 ift57hi3417Tg/hi3417Tg standard conditions Pec-fin whole organism WB
Ab1-ift52 ift88tz288/tz288 standard conditions Pec-fin whole organism WB
ift88 Ab1-ift88 WT standard conditions Pec-fin whole organism WB
Ab1-ift88 ift172hi2211Tg/hi2211Tg standard conditions Pec-fin whole organism WB
Ab1-ift88 ift57hi3417Tg/hi3417Tg standard conditions Pec-fin whole organism WB
Ab1-ift88 ift88tz288/tz288 standard conditions Pec-fin Not Detected whole organism WB
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Vision Research for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Vision Research, 49(4), Sukumaran, S., and Perkins, B.D., Early defects in photoreceptor outer segment morphogenesis in zebrafish ift57, ift88 and ift172 Intraflagellar Transport mutants, 479-489, Copyright (2009) with permission from Elsevier. Full text @ Vision Res.