ZFIN ID: ZDB-FIG-090306-9
Sukumaran et al., 2009 - Early defects in photoreceptor outer segment morphogenesis in zebrafish ift57, ift88 and ift172 Intraflagellar Transport mutants. Vision Research   49(4):479-489 Full text @ Vision Res.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Term:
Stage: Protruding-mouth

Fig. 8 Western blot analysis of whole wild type and IFT mutant embryos at 72 hpf. Immunoreactivity against IFT52 (top row) were detected in wild type and mutants at 72 hpf, confirming the expression of these subunits. IFT88 immunoreactivity (middle row) was not seen in the ift88 mutants but continued to be expressed in other mutants as well as wild type. Acetylated tubulin (AT) served as a control (bottom row).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ift52 Ab1-ift52 WT standard conditions Protruding-mouth whole organism WB
Ab1-ift52 ift172hi2211Tg/hi2211Tg standard conditions Protruding-mouth whole organism WB
Ab1-ift52 ift57hi3417Tg/hi3417Tg standard conditions Protruding-mouth whole organism WB
Ab1-ift52 ift88tz288/tz288 standard conditions Protruding-mouth whole organism WB
ift88 Ab1-ift88 WT standard conditions Protruding-mouth whole organism WB
Ab1-ift88 ift172hi2211Tg/hi2211Tg standard conditions Protruding-mouth whole organism WB
Ab1-ift88 ift57hi3417Tg/hi3417Tg standard conditions Protruding-mouth whole organism WB
Ab1-ift88 ift88tz288/tz288 standard conditions Protruding-mouth Not Detected whole organism WB
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Vision Research for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Vision Research, 49(4), Sukumaran, S., and Perkins, B.D., Early defects in photoreceptor outer segment morphogenesis in zebrafish ift57, ift88 and ift172 Intraflagellar Transport mutants, 479-489, Copyright (2009) with permission from Elsevier. Full text @ Vision Res.