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Fig. 6 Western blot analysis of whole wild type and IFT mutant embryos at 60 hpf. Immunoreactivity against IFT52 protein (top row) was detected in wild type, ift57, ift88, and ift172 mutants. Immunoreactivity against IFT88 protein (middle row) was seen only in wild type, ift57 and ift172 mutants, thus showing that the ift88 mutant is null. Acetylated tubulin (AT) served as a control (bottom row).
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Reprinted from Vision Research, 49(4), Sukumaran, S., and Perkins, B.D., Early defects in photoreceptor outer segment morphogenesis in zebrafish ift57, ift88 and ift172 Intraflagellar Transport mutants, 479-489, Copyright (2009) with permission from Elsevier. Full text @ Vision Res.