FIGURE

Fig. 5

ID

ZDB-FIG-090306-6

Publication

Sukumaran et al., 2009 - Early defects in photoreceptor outer segment morphogenesis in zebrafish ift57, ift88 and ift172 Intraflagellar Transport mutants

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Fig. 5

Immunohistochemical analysis of wild type, ift57, ift88, and ift172 mutant zebrafish at 60 hpf. (A–D) 1D1 (green), a marker for rhodopsin, localized to the outer segment region in wild type and ift57 mutants. Strong mislocalization to the inner segment was also observed in ift57 mutants, as well as ift88, and ift172 mutants. (E–H) Zpr1 (green), a label for red/green double cones gave an elongated, columnar morphology in wild type animals. Cone morphology was disheveled in the three mutants. (I–J) Blue opsin (BOPS) was seen in small foci of wild type photoreceptors. Similar staining was seen in ift57, ift88, and ift172 mutants. (M–P) IFT52 (red) co-localized with acetylated tubulin (green) in the connecting cilia of wild type animals (arrows). No such staining was observed in ift57, ift88, and ift172 mutants. (Q–T) IFT88 (red) also showed apical localization in wild type animals (arrows). The staining partially overlapped with acetylated tubulin (green). No such staining was observed in IFT mutants. (U–X) The basal body marker centrin (red) localized to the apical surface of the inner segment. Acetylated tubulin (green) denotes microtubules. Centrin was seen in wild type and all IFT mutants (arrows). In all images, the tissues were counterstained with DAPI (blue). Scale bar = 20 μm.

Expression Data

Antibodies:	Ab1-ift52 Ab1-ift88 Ab1-opn1sw2 Ab1-rho zpr-1
Fish:	WT ift57^{hi3417Tg/hi3417Tg} ift88^tz288/tz288 ift172^{hi2211Tg/hi2211Tg}
Anatomical Terms:	eye photoreceptor cell eye photoreceptor cell photoreceptor connecting cilium eye photoreceptor cell photoreceptor inner segment eye photoreceptor cell photoreceptor outer segment retinal cone cell
Stage:	Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ift57^{hi3417Tg/hi3417Tg} ift88^tz288/tz288 ift172^{hi2211Tg/hi2211Tg}
Observed In:	retinal cone cell
Stage:	Pec-fin

Phenotype Detail

Acknowledgments

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Reprinted from Vision Research, 49(4), Sukumaran, S., and Perkins, B.D., Early defects in photoreceptor outer segment morphogenesis in zebrafish ift57, ift88 and ift172 Intraflagellar Transport mutants, 479-489, Copyright (2009) with permission from Elsevier. Full text @ Vision Res.