FIGURE

Fig. S2

ID

ZDB-FIG-090113-65

Publication

Walters et al., 2009 - Muscle degeneration and leukocyte infiltration caused by mutation of zebrafish fad24

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Fig. S2

Neutrophils in fad24hi1019 larvae respond to wounds and have active MPO contained in cytoplasmic granules. A: A fad24hi1019;mpx:GFP mutant at 3 dpf was analyzed by time-lapse confocal microscopy. Frames are taken from Supp. Movie 3. The asterisk indicates a wound made in the dorsal fin. A single neutrophil is marked in each frame in green with a red track indicating the path it takes from the body to the fin. Indicated times are from the start of the movie. B: A blown-up image of the wound (taken from Supp. Movie 4) reveals the presence of a low-level MPO-expressing cell with a long and extended morphology (arrowhead). C: Both a low-level MPO-expressing cell with an extended morphology (arrowhead) and a high-level MPO-expressing neutrophil (arrow) can be compared at the wound. Asterisk indicates the wound. D,E: The differential subcellular localization of MPO (D) and L-plastin (E) can be seen in a confocal composite image of two neutrophils from a co-immunolabled fad24hi1019 larva. F,F′: Whole-mount endogenous MPO activity in WT (F) and fad24hi1019 (F′) larvae. Representative results from at least 3 experiments.

Expression Data

Genes:	lcp1 mpx
Antibodies:	Ab1-lcp1 Ab1-mpx
Fish:	noc3l^hi1019Tg/+; uwm1Tg
Anatomical Term:	neutrophil
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	noc3l^{hi1019Tg/hi1019Tg}
Observed In:	peroxidase activity
Stage:	Unknown

Phenotype Detail

Acknowledgments

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