PUBLICATION

Muscle degeneration and leukocyte infiltration caused by mutation of zebrafish fad24

Authors
Walters, K.B., Dodd, M.E., Mathias, J.R., Gallagher, A.J., Bennin, D.A., Rhodes, J., Kanki, J.P., Look, A.T., Grinblat, Y., and Huttenlocher, A.
ID
ZDB-PUB-090106-2
Date
2009
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   238(1): 86-99 (Journal)
Registered Authors
Dodd, M. Ernest, Gallagher, Andrea, Grinblat, Yevgenya, Huttenlocher, Anna, Kanki, John, Look, A. Thomas, Mathias, Jonathan, Rhodes, Jennifer, Walters, Kevin
Keywords
zebrafish, fad24, neutrophil, inflammation
MeSH Terms
  • Mutation*
  • Lipid Metabolism
  • Humans
  • Basic-Leucine Zipper Transcription Factors/genetics
  • Basic-Leucine Zipper Transcription Factors/metabolism*
  • Muscular Atrophy*/genetics
  • Muscular Atrophy*/pathology
  • Apoptosis/physiology
  • Oligonucleotides, Antisense/metabolism
  • Zebrafish*/anatomy & histology
  • Zebrafish*/physiology
  • In Situ Hybridization
  • Muscle, Skeletal/cytology
  • Muscle, Skeletal/metabolism
  • Muscle, Skeletal/pathology
  • Basic Helix-Loop-Helix Transcription Factors/genetics
  • Basic Helix-Loop-Helix Transcription Factors/metabolism*
  • Nuclear Proteins/genetics
  • Nuclear Proteins/metabolism*
  • Animals
  • Leukocytes/metabolism*
  • Cell Nucleus/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
(all 24)
PubMed
19097072 Full text @ Dev. Dyn.
Abstract
Factor for adipocyte differentiation 24 (fad24) is a novel gene that has been implicated in adipocyte differentiation and DNA replication. In a screen for zebrafish mutants that have an abnormal tissue distribution of neutrophils, we identified an insertional allele of fad24, fad24(hi1019). Homozygous fad24(hi1019) larvae exhibit muscle degeneration accompanied by leukocyte infiltration. Muscle degeneration was extensive and included tissue apoptosis and disorganized, poorly striated muscle fibers. Blocking apoptosis using pan-caspase inhibitors resulted in decreased neutrophil recruitment into the body of the larva, suggesting a causative link between apoptosis and leukocyte infiltration. These findings suggest that zebrafish is a powerful genetic model system to address the interplay between muscle degeneration and leukocyte infiltration, and indicate that tissue apoptosis may contribute to neutrophil recruitment in some inflammatory states. Developmental Dynamics 238:86-99, 2009. (c) 2008 Wiley-Liss, Inc.
Genes / Markers
Figures
Figure Gallery (11 images) / 2
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WT + MO1-noc3lstandard conditionsProtruding-mouth
WT + MO1-noc3lstandard conditionsProtruding-mouth
WT + MO1-noc3lstandard conditionsProtruding-mouth
WT + MO1-noc3lstandard conditionsProtruding-mouth
WT + MO1-noc3lstandard conditionsProtruding-mouth
WT + MO1-noc3lstandard conditionsProtruding-mouth
WT + MO1-spi1bstandard conditionsProtruding-mouth
WT + MO1-spi1bstandard conditionsProtruding-mouth
WT + MO2-noc3lstandard conditionsProtruding-mouth
WT + MO2-noc3lstandard conditionsProtruding-mouth
1 - 10 of 42
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
hi1019TgTransgenic Insertion
uwm1TgTransgenic Insertion
    1 - 2 of 2
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    noc3lMO1-noc3lMRPHLNO
    noc3lMO2-noc3lMRPHLNO
    spi1bMO1-spi1bMRPHLNO
    1 - 3 of 3
    Show
    Fish
    Fish
    noc3lhi1019Tg/hi1019Tg
    noc3lhi1019Tg/hi1019Tg + MO1-spi1b
    noc3lhi1019Tg/+; uwm1Tg
    WT
    WT + MO1-noc3l
    WT + MO1-spi1b
    WT + MO2-noc3l
    1 - 7 of 7
    Show
    Antibodies
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    GFPEFGGFP
    1 - 1 of 1
    Show
    Mapping
    No data available
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    Citation
    Walters, K.B., Dodd, M.E., Mathias, J.R., Gallagher, A.J., Bennin, D.A., Rhodes, J., Kanki, J.P., Look, A.T., Grinblat, Y., and Huttenlocher, A. (2009) Muscle degeneration and leukocyte infiltration caused by mutation of zebrafish fad24. Developmental Dynamics : an official publication of the American Association of Anatomists. 238(1):86-99.