FIGURE

Fig. 3

ID

ZDB-FIG-090109-24

Publication

Rai et al., 2008 - DNA demethylation in zebrafish involves the coupling of a deaminase, a glycosylase, and gadd45

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Fig. 3

A PCR Strategy to Detect a G:T Intermediate

(A) Schematic of the PCR reaction for thymine (C^meCGG > CTGG) detection at M-DNA HpaII/MspI sites using an A-tailed primer (only 3 of the ∼22 bases shown) with an adenosine at the 3′ end.

(B) Detection of a G:T mismatch on M-DNA by PCR. M-DNA, AID mRNA, and RNA encoding either wild-type or catalytically inactive hMbd4 (D560A) was injected at the single-cell stage and assessed at 13 hpf.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 135(7), Rai, K., Huggins, I.J., James, S.R., Karpf, A.R., Jones, D.A., and Cairns, B.R., DNA demethylation in zebrafish involves the coupling of a deaminase, a glycosylase, and gadd45, 1201-1212, Copyright (2008) with permission from Elsevier. Full text @ Cell