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Fig. S3

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ZDB-FIG-090109-27

Publication

Rai et al., 2008 - DNA demethylation in zebrafish involves the coupling of a deaminase, a glycosylase, and gadd45

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Fig. S3

Efficiency of morpholino knockdown of deaminase proteins and MBD4
(A-B) Morpholino knockdown efficiency of Apobec2a (4pg) (A) and Apobec2b (2pg) (B) was assessed at protein level by western blotting using whole cell extracts derived from wild type or morpholino-injected embryos. Anti-Apobec2a and anti-Apobec2b were specific rabbit antibodies generated from recombinant zebrafish proteins. Anti-Vinculin and anti-panH3 antibodies were used as loading controls. In panel B arrow indicates the band for Apobec2b whereas asterisk indicates a non-specific band. (C-D) Morpholino knockdown of AID and MBD4 was assessed at 80% epiboly in cDNA made from total RNA obtained from wild type embryos or embryos injected with AID Mo (4 pg) or MBD4 Mo (4pg). Spliced and unspliced DNA length is shown. 28S was used as a loading control.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 135(7), Rai, K., Huggins, I.J., James, S.R., Karpf, A.R., Jones, D.A., and Cairns, B.R., DNA demethylation in zebrafish involves the coupling of a deaminase, a glycosylase, and gadd45, 1201-1212, Copyright (2008) with permission from Elsevier. Full text @ Cell