FIGURE

Fig. S7

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ZDB-FIG-090109-31

Publication

Rai et al., 2008 - DNA demethylation in zebrafish involves the coupling of a deaminase, a glycosylase, and gadd45

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Fig. S7

Physical interactions between AID and Mbd4 and enhancement by Gadd45α
Western blot showing the co-immunoprecipitation of V5-AID and Myc-hMbd4 (A-B), V5-Apobec2a and Myc-hMbd4 (C-D) and HA-Apobec2b and Myc-MBD4 (E-F) in the absence (A, C, E) or presence (B,D,F) of Gadd45α when overexpressed in RKO cells. (G-J) Interaction between Myc-hMbd4 and HA-tagged Gadd45α (G), V5-AID and HAGadd45α (H), V5-Apobec2a and HA-Gadd45α (I) and HA-Apobec2b and His-Gadd45α (J) were detected in RKO cell extracts overexpressing the two proteins, suggesting that Gadd45α can individually interact with deaminases or hMbd4. Immune complexes were pulled down using the IP antibody as shown, and interacting proteins were detected in western blot format (WB) antibody as shown.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 135(7), Rai, K., Huggins, I.J., James, S.R., Karpf, A.R., Jones, D.A., and Cairns, B.R., DNA demethylation in zebrafish involves the coupling of a deaminase, a glycosylase, and gadd45, 1201-1212, Copyright (2008) with permission from Elsevier. Full text @ Cell