FIGURE

Fig. 4

ID

ZDB-FIG-081103-41

Publication

Leung et al., 2008 - The orphan G protein-coupled receptor 161 is required for left-right patterning

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Fig. 4

(A) bmp4 expression was predominantly on the left side of the cardiac cone in comparison to the uniform expression of cmlc2 (inset) which was disrupted in gpr161 knockdown embryos. Graphical summary of control (85.8 ± 3.7%; n = 254), gpr161 knockdown (MO#24, 47.5 ± 5.7%; n = 193) and t-test (p < 0.01) results were from 7 injection experiments. (B) bmp4 expression was predominantly on the left side of the heart tube in comparison to the uniform expression of cmlc2 (inset) was disrupted in gpr161 knockdown embryos. Graphical summary of control (99.5 ± 1.3%; n = 141), gpr161 knockdown (MO#24, 56.9 ± 5.9%; n = 102) and t-test (p < 0.01) results were from 4 injection experiments. Dashed lines were drawn over the cardiac cone and heart tube and the midline of the structures. n was number of total embryos. Error bars were ± SEM. All scale bars were 100 μm.

Expression Data

Genes:	bmp4 myl7
Fish:	WT WT + MO1-gpr161b
Knockdown Reagent:	MO1-gpr161b
Anatomical Terms:	heart rudiment heart tube
Stage Range:	26+ somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-gpr161b
Knockdown Reagent:	MO1-gpr161b
Observed In:	determination of left/right symmetry heart morphogenesis heart rudiment heart tube
Stage Range:	26+ somites to Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 323(1), Leung, T., Humbert, J.E., Stauffer, A.M., Giger, K.E., Chen, H., Tsai, H.J., Wang, C., Mirshahi, T., and Robishaw, J.D., The orphan G protein-coupled receptor 161 is required for left-right patterning, 31-40, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.