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ZFIN ID: ZDB-FIG-081103-39
Leung et al., 2008 - The orphan G protein-coupled receptor 161 is required for left-right patterning. Developmental Biology   323(1):31-40 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-25 to Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-25 to Day 4

Fig. 2 gpr161 knockdown disrupts cardiac looping morphogenesis. (A) Lateral view of zebrafish hearts at 4 dpf. Control and gpr161 knockdown in transgenic zebrafish embryos with cardiac specific GFP, TG[cmlc2:GFP], showing images of bright field (top panels) and fluorescent (bottom panels). A* labelled atrium, V* labelled ventricle. (B) Cardiac looping morphogenesis marked by cmlc2 expression in control (D-looping), gpr161 knockdown (unlooped and L-looping) and RNA rescued embryos. All scale bars were 100 μm. (C) Graphical summary of gpr161 knockdown disrupted normal cardiac D-looping morphogenesis at 36 hpf. Calculation as % of embryos with D-looping in control (97.8 ± 1.3%; n = 223), embryos injected with gpr161 morpholino (MO#36, 32.4 ± 4.1%; n = 293) and RNA rescue (gpr161 RNA without 5′UTR; 52.2 ± 3.4%; n = 157), n was number of total embryos and results were from 4 injection experiments. Error bars were ± SEM. t-test * and # indicated statistical significance, p < 0.01.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP twu34Tg standard conditions Day 4 atrium IFL
Day 4 cardiac ventricle IFL
twu34Tg + MO2-gpr161 standard conditions Day 4 atrium IFL
Day 4 cardiac ventricle IFL
myl7 WT standard conditions Prim-25 presumptive atrium heart tube ISH
Prim-25 presumptive cardiac ventricle heart tube ISH
WT + MO2-gpr161 standard conditions Prim-25 presumptive atrium heart tube ISH
Prim-25 presumptive cardiac ventricle heart tube ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-gpr161 standard conditions Prim-25 cardiac ventricle orientation atrium, abnormal
Prim-25 heart looping disrupted, abnormal
twu34Tg + MO2-gpr161 standard conditions Day 4 cardiac ventricle orientation atrium, abnormal
twu34Tg + MO2-gpr161 standard conditions Day 4 heart looping disrupted, abnormal
twu34Tg + MO2-gpr161 standard conditions Day 4 pericardium edematous, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 323(1), Leung, T., Humbert, J.E., Stauffer, A.M., Giger, K.E., Chen, H., Tsai, H.J., Wang, C., Mirshahi, T., and Robishaw, J.D., The orphan G protein-coupled receptor 161 is required for left-right patterning, 31-40, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.