ZFIN ID: ZDB-FIG-081028-14
Ahn et al., 2008 - Tri-phasic expression of posterior Hox genes during development of pectoral fins in zebrafish: Implications for the evolution of vertebrate paired appendages. Developmental Biology   322(1):220-233 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25

Fig. 4 Early expression of hoxab cluster genes in zebrafish pectoral fins. Expression of hoxa9b starts within the mesenchyme cells of anterior fin field (lying lateral to the second myotome) and subsequently spreads toward the posterior, eventually encompassing the entire fin bud mesenchyme by 30 hpf (A–C: phase I expression). For hoxa10b and hoxa11b, the initial expression is confined to the myogenic mesenchyme cells (E–G, I–K). For these genes, expression within the chondrogenic mesenchyme cells begins around 30 hpf in distal posterior cells (phase II expression) roughly overlying the medial group of myogenic cells (H, L: arrows). In dorsal views, this creates a misleading impression of enhanced expression in the medial cluster of prospective pectoral fin muscle cells (compare Figs. 4H, L with Figs. 2D, I). Oblique dorsal views with anterior to the left in all panels. Each embryo is also counter-stained for muscle myosin (brown) to show the position of myotomes. hpf: hours post fertilization.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
hoxa9b WT standard conditions Prim-5 chondroblast ISH
Prim-5 mesenchyme ISH
Prim-5 pectoral fin bud ISH
Prim-15 chondroblast ISH
Prim-15 mesenchyme ISH
Prim-15 pectoral fin bud ISH
Prim-25 chondroblast ISH
Prim-25 mesenchyme pectoral fin ISH
hoxa10b WT standard conditions Prim-5 mesenchyme ISH
Prim-5 myoblast ISH
Prim-5 pectoral fin bud ISH
Prim-15 chondroblast ISH
Prim-15 mesenchyme ISH
Prim-15 myoblast ISH
Prim-15 pectoral fin bud ISH
Prim-25 chondroblast ISH
Prim-25 mesenchyme pectoral fin ISH
Prim-25 myoblast ISH
hoxa11b WT standard conditions Prim-5 mesenchyme ISH
Prim-5 myoblast ISH
Prim-5 pectoral fin bud ISH
Prim-15 chondroblast ISH
Prim-15 mesenchyme ISH
Prim-15 myoblast ISH
Prim-15 pectoral fin bud ISH
Prim-25 chondroblast ISH
Prim-25 mesenchyme pectoral fin ISH
Prim-25 myoblast ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-A4.1025 IHC WT standard conditions Prim-5 - Prim-25 myotome muscle myosin complex
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 322(1), Ahn, D., and Ho, R.K., Tri-phasic expression of posterior Hox genes during development of pectoral fins in zebrafish: Implications for the evolution of vertebrate paired appendages, 220-233, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.