FIGURE

Fig. 5

ID

ZDB-FIG-081010-12

Publication

Gansner et al., 2008 - Essential role for fibrillin-2 in zebrafish notochord and vascular morphogenesis

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Fig. 5

pfd^gw1 mutants are sensitized to notochord distortion after partial knockdown of loxl5b. A-D: Embryos from pfd^gw1/+ intercrosses were injected with 5 ng of control MO (A,B) or 5 ng loxl5b MO (C,D) and examined at 30 hours postfertilization (hpf). Neither morpholino induced notochord distortion in wild-type embryos (A,C). However, pfd^gw1 mutants injected with lysyl oxidase morpholino developed striking notochord distortion (D, arrowheads) compared with mutants injected with control morpholino (B, arrowheads). E: Wild-type embryo incubated in high-dose (6 μM) neocuproine, demonstrating classic sine-wave appearance of lysyl oxidase inhibition at 30 hpf. F: pfd^gw1 mutant at 30 hpf with irregular notochord distortion after incubation in 6 μM neocuproine. Embryos were treated with PTU to inhibit melanin pigmentation.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fbn2b^gw1/gw1 (AB) AB + MO1-loxl5b fbn2b^gw1/gw1 + MO1-loxl5b (AB)
Knockdown Reagent:	MO1-loxl5b
Observed In:	notochord
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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