FIGURE

Fig. 2

ID
ZDB-FIG-080918-36
Publication
Tobin et al., 2008 - Inhibition of neural crest migration underlies craniofacial dysmorphology and Hirschsprung's disease in Bardet-Biedl syndrome
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Fig. 2

Craniofacial and neural crest defects in bbs zebrafish morphants. (A–E) Ventral views of wholemount, Alcian Blue-stained, 5-dpf zebrafish. bbs8 morphants are most severe (D), with loss of pharyngeal apparatus (2D arrowhead), mandibles, and shortening of the chondrocranium. (F–J) Lateral views of larvae showing reduction of branchial arches and shortening of the mandibles in bbs6 and bbs8 morphants. (K–O) Flatmounts of neurocrania of controls and morphants. bbs8 morphants often have fusion of the trabeculae at the midline (arrowhead) similar to syu mutants. (P–T) Crestin in situ in 20ss embryos reveals streams of migrating NCCs in controls (arrowhead) with progressively fewer streams in bbs4, bbs6, and bbs8 morphants, respectively. (U–X) Sox10:eGFP expression in migrating streams (arrowheads) shows a similar reduction in the number of streams to the crestin expression. (Y) Quantification of the number of streams in morphants confirms a severe neural crest migration defect in bbs8 morphants that can be rescued by human mRNA. (Z and AA) dct expression in 27 hpf control embryos (Z) and bbs8 morphants (AA). [Scale bars: 500 μm (A–J), 100 μm (K–O), and 100 μm (P–X).]

Expression Data
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 5

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA