ZFIN Figure: Fish et al., 2008, Fig. 3

Fig. 3

miR-126 Regulates Vascular Integrity and Lumen Maintenance In Vivo

(A) miR-126 and miR-126^* enrichment (qRT-PCR) in GFP⁺ endothelial cells from 72 hpf Tg(flk1:GFP)^s843 zebrafish compared to GFP^- cells.

(B) Relative levels of miR-126 and miR-126^* in 72 hpf zebrafish embryos.

(C) miR-126 expression monitored by qRT-PCR during zebrafish development.

(D) Levels of miR-126/126^* or egfl7 (across intron containing miR-126) quantified by qRT-PCR in 72 hpf zebrafish injected with miR-126 MOs relative to control.

(E) Lateral views of control and miR-126 MO-injected Tg(flk1:GFP)^s843; Tg(gata1:dsRed)^sd2 zebrafish (72 hpf). Brightfield microscopy (top) revealed no major changes in gross morphology, while flk1:GFP showed normal blood vessel patterning (middle). Presence of blood cells (gata1:dsRed) in the intersomitic vessels (isv), dorsal aorta (da) and primary cardinal vein (pcv) was greatly reduced in morphants (bottom). y, yolksac; h, head; ht, heart.

(F) miR-126 morphants (48 hpf) had normal vessel patterning (flk1:GFP), but developed cranial hemorrhages (gata1:dsRed; arrow) in the head. baa, branchial arch arteries.

(G) Ventral view of baa suggested smaller luminal size in miR-126 morphants.

(H) Transverse section of control or miR-126 MO-treated zebrafish revealed that the da and pcv of morphants had a smaller lumen than controls; higher magnification (right panels) of boxed area shows collapsed da and small pcv in morphants. flk1:GFP, green; ZO-1, an epithelial marker, red.

Expression Data

Genes:	DsRed EGFP tjp1a
Fish:	s843Tg; sd2Tg s843Tg; sd2Tg + MO1-mir126 + MO2-mir126a
Knockdown Reagents:	MO1-mir126 MO2-mir126a
Anatomical Terms:	cranial vasculature dorsal aorta intersegmental vessel nucleate erythrocyte pharyngeal vasculature posterior cardinal vein
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s843Tg; sd2Tg + MO1-mir126 + MO2-mir126a
Knockdown Reagents:	MO1-mir126 MO2-mir126a
Observed In:	blood blood circulation blood vasculature blood vessel morphogenesis cranial vasculature dorsal aorta nucleate erythrocyte pharyngeal vasculature posterior cardinal vein
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 15(2), Fish, J.E., Santoro, M.M., Morton, S.U., Yu, S., Yeh, R.F., Wythe, J.D., Ivey, K.N., Bruneau, B.G., Stainier, D.Y., and Srivastava, D., miR-126 regulates angiogenic signaling and vascular integrity, 272-284, Copyright (2008) with permission from Elsevier. Full text @ Dev. Cell