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ZIRC
ZFIN ID: ZDB-FIG-080829-20
Grosskurth et al., 2008 - Emergence of Xin demarcates a key innovation in heart evolution. PLoS One   3(8):e2857 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage: Adult

Fig. 6 Immunofluorescence microscopy of zebrafish and chicken heart sections.
(A–F) Double-label indirect immunofluorescence was performed on frozen sections of zebrafish heart with monoclonal anti-troponin T or anti-plakoglobin (green, visualized by fluorescein-conjugated 2nd antibody) and polyclonal anti-mXin (red, visualized by rhodamine-conjugated 2nd antibody). Before mounting, the sections were treated with DAPI to stain nuclei (blue). Scale bar: 10 μm. (G–I) Double-label indirect immunofluorescence was performed on chicken heart section with monoclonal anti-N-cadherin (green) and polyclonal anti-mXin antibody (red). Scale bar: 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
jupa Ab1-jup WT standard conditions Adult heart intercalated disc IHC
tnnt2a Ab1-tnnt2 WT standard conditions Adult heart muscle thin filament tropomyosin IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One