Immunofluorescence microscopy of zebrafish and chicken heart sections. (A–F) Double-label indirect immunofluorescence was performed on frozen sections of zebrafish heart with monoclonal anti-troponin T or anti-plakoglobin (green, visualized by fluorescein-conjugated 2nd antibody) and polyclonal anti-mXin (red, visualized by rhodamine-conjugated 2nd antibody). Before mounting, the sections were treated with DAPI to stain nuclei (blue). Scale bar: 10 μm. (G–I) Double-label indirect immunofluorescence was performed on chicken heart section with monoclonal anti-N-cadherin (green) and polyclonal anti-mXin antibody (red). Scale bar: 10 μm.
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