FIGURE

Fig. 4

ID
ZDB-FIG-080829-18
Publication
Grosskurth et al., 2008 - Emergence of Xin demarcates a key innovation in heart evolution
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Fig. 4

Western blot analysis of protein extracts prepared from mouse, turkey, chicken, frog and zebrafish hearts with polyclonal U1013 anti-Xin antibody.
As previously reported [5], the U1013 antibody generated against the N-terminal fragment of mXinα including the Xin repeat region specifically reacts with mXinα (155 kDa), mXinα-a (250 kDa) and mXinβ (∼340 kDa) from mouse heart. Similarly, this antibody recognizes a 217 kDa band (indicated by *), and 280–295 kDa bands (indicated by**) from turkey, chicken, frog and zebrafish heart extracts. These bands may represent Xinα and its splicing variants Xinα-a. Many degraded fragments were also detected by this antibody. In frog and zebrafish but not turkey and chicken heart extracts, this antibody also reacts with a 335 kDa band (indicated by ***), which may represent Xinβ isoform.

Expression Data
Genes:
Antibody:
Fish:
Anatomical Term:
Stage: Adult

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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