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ZFIN ID: ZDB-FIG-080723-2
Boisset et al., 2008 - Characterization of pip5k3 fleck corneal dystrophy-linked gene in zebrafish. Gene expression patterns : GEP   8(6):404-410 Full text @ Gene Expr. Patterns
Anatomical Terms:
Stage Range: 4-cell to Days 7-13

Fig. 3 Embryonic expression of zebrafish pip5k3. (I) RT-PCR analysis of embryonic pip5k3 during development. Pip5k3 transcripts were detected at 2 hpf, 1 dpf, 5 dpf and 10 dpf. Absence of DNA contamination was controlled by a no reverse transcriptase experiment. The amplified fragments lengths are written on the right. (II) Whole mount in situ hybridization. (A) Control experiments using sense probe. (B, C, dorsal view and D, lateral view) pip5k3 was uniformly expressed at 4-cell stage, 18 somites and 24 hpf. (E, G and I) Expression of pip5k3 in the head region at 48 hpf, 72 hpf and 5 dpf. Note that the staining in the gut region was not specific compared to the sense probe (I and data not shown). (F, H and J) View of the tail of 48 hpf, 72 hpf and 5 dpf embryos. No staining was detected at 48 hpf in contrast to 72 hpf and 5 dpf where a partial expression of pip5k3 was found in somites. Note that the blue spot localized to the proctodeum at 5 dpf was an artefact (J). (K) In situ on 12 μm cryosectionned eye of 3 dpf-old larva. Staining was mainly observed in OLM, INL, GCL and L. (L) Eye of 5 dpf-old larva showing expression of pip5k3 in INL, GCL, L and C. OLM, outer limiting membrane; INL, inner nuclear layer; GCL, ganglion cell layer; L, lens; C, cornea. Scale bars: (A and B) 20 μm; (C–H and J) 100 μm; (I) 200 μm; (K and L) 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
pikfyve AB standard conditions 4-cell whole organism ISH
64-cell whole organism RTPCR
14-19 somites whole organism ISH
Prim-5 whole organism RTPCR
Prim-5 whole organism ISH
Long-pec head ISH
Long-pec inner ear ISH
Long-pec Not Detected somite ISH
Protruding-mouth head ISH
Protruding-mouth lens ISH
Protruding-mouth outer limiting membrane ISH
Protruding-mouth retinal ganglion cell layer ISH
Protruding-mouth retinal inner nuclear layer ISH
Protruding-mouth trunk ISH
Day 5 cornea ISH
Day 5 head ISH
Day 5 lens ISH
Day 5 retinal ganglion cell layer ISH
Day 5 retinal inner nuclear layer ISH
Day 5 trunk ISH
Day 5 whole organism RTPCR
Days 7-13 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Gene expression patterns : GEP for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Gene expression patterns : GEP, 8(6), Boisset, G., Polok, B.K., and Schorderet, D.F., Characterization of pip5k3 fleck corneal dystrophy-linked gene in zebrafish, 404-410, Copyright (2008) with permission from Elsevier. Full text @ Gene Expr. Patterns