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ZFIN ID: ZDB-FIG-080723-1
Boisset et al., 2008 - Characterization of pip5k3 fleck corneal dystrophy-linked gene in zebrafish. Gene expression patterns : GEP   8(6):404-410 Full text @ Gene Expr. Patterns
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Adult

Fig. 2 Expression profile of adult zebrafish pip5k3. (I) RT-PCR analysis of pip5k3 expression in different tissues of zebrafish. Pip5k3 was expressed in all tested tissues, but at low level in swim bladder. The house-keeping gene actin was used as control. The size of the amplified fragments is indicated on the right. (II, A–H) In situ hybridization using RNA probe specific for zebrafish pip5k3 on adult eye, brain and muscle cryosections. (A) Pip5k3 was expressed in the cornea and lens. (C) In the retina, pip5k3 staining was seen in GCL, INL and OLM. (E) In the brain, pip5k3 staining was mainly observed in PGZ, Val and TL. (G) Pip5k3 staining was perinuclear and located to the membranes of the cells in the muscle. (B, D, F and H) Control staining with sense probe. C, cornea; L, lens; GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; LT, longitudinal torus; OLM, outer limiting membrane; ONL, outer nuclear layer; OPL, outer plexiform layer; OT, optic tectum; PGZ, periventricular gray zone of the optic tectum; Val, lateral division of valvula cerebelli. Scale bars: (A and B) 100 μm; (C and D and G and H) 10 μm; (E and F) 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
pikfyve AB standard conditions Adult brain RTPCR
Adult cornea ISH
Adult eye RTPCR
Adult gut RTPCR
Adult heart RTPCR
Adult integument RTPCR
Adult intestine RTPCR
Adult lateral valvula cerebelli ISH
Adult lens ISH
Adult liver RTPCR
Adult muscle RTPCR
Adult muscle cell ISH
Adult outer limiting membrane ISH
Adult ovary RTPCR
Adult periventricular grey zone ISH
Adult retinal ganglion cell layer ISH
Adult retinal inner nuclear layer ISH
Adult swim bladder RTPCR
Adult torus longitudinalis ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Gene expression patterns : GEP for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Gene expression patterns : GEP, 8(6), Boisset, G., Polok, B.K., and Schorderet, D.F., Characterization of pip5k3 fleck corneal dystrophy-linked gene in zebrafish, 404-410, Copyright (2008) with permission from Elsevier. Full text @ Gene Expr. Patterns