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ZFIN ID: ZDB-FIG-080617-9
Anderson et al., 2008 - Loss of unc45a precipitates arteriovenous shunting in the aortic arches. Developmental Biology   318(2):258-267 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 4

Fig. 4 Increased distance between left and right posterior ceratobranchial cartilages is a specific kustr12 phenotype. Aortic arches (bright green) develop in close association with pharyngeal cartilages (red) in WT (A) and kustr12 (D). Note the malformed ventral aorta (VA) in kustr12. (The darker green staining is background and represents pharyngeal muscle.) Defects in jaw and pharyngeal cartilages in kustr12 include shortening of the palatoquadrates (PQ); compression of the basibranchial cartilages (horizontal white line); improper angling of the ceratobranchial (asterisks) and ceratohyal (arrowheads) cartilages; and decreased lateral distance (vertical white lines) between left and right ceratobranchial cartilages in pharyngeal arches 5 and 6 [compare B (WT) to E (kustr12)]. Like kustr12, vbgy6 mutants (C) and tnnt2 morphants (F) develop edema and exhibit all described cartilage defects except increased lateral distance between posterior ceratobranchials. Thus, only the latter defect is likely a direct effect of the kustr12 mutation. A, D: 2D confocal projections of TG(flk1:GFPla116; gata1:dsRed) embryos stained with anti-collagen II (red) and anti-GFP (green). B, C, E, F: Macro images of embryos stained with anti-collagen II. All embryos are 4 dpf; ventral views, anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP Ab4-GFP la116Tg; sd2Tg standard conditions Day 4 aortic arch IHC
Ab4-GFP Day 4 blood vessel endothelial cell IHC
Ab4-GFP Day 4 ventral aorta IHC
Ab4-GFP unc45atr12/tr12; la116Tg; sd2Tg standard conditions Day 4 aortic arch IHC
Ab4-GFP Day 4 blood vessel endothelial cell IHC
Ab4-GFP Day 4 ventral aorta IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-col2a IHC la116Tg; sd2Tg standard conditions Day 4 ceratobranchial cartilage
IHC Day 4 ceratohyal cartilage
IHC Day 4 palatoquadrate cartilage
IHC Day 4 pharyngeal arch cartilage
IHC unc45atr12/tr12; la116Tg; sd2Tg standard conditions Day 4 ceratobranchial cartilage
IHC Day 4 ceratohyal cartilage
IHC Day 4 palatoquadrate cartilage
IHC Day 4 pharyngeal arch cartilage
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tnnt2a standard conditions Day 4 basibranchial condensed, abnormal
Day 4 ceratobranchial cartilage orientation ceratobranchial cartilage, abnormal
Day 4 ceratohyal cartilage orientation ceratohyal cartilage, abnormal
Day 4 palatoquadrate arch decreased length, abnormal
acvrl1y6/y6 standard conditions Day 4 basibranchial condensed, abnormal
Day 4 ceratobranchial cartilage orientation ceratobranchial cartilage, abnormal
Day 4 ceratohyal cartilage orientation ceratohyal cartilage, abnormal
Day 4 palatoquadrate arch decreased length, abnormal
unc45atr12/tr12 standard conditions Day 4 basibranchial condensed, abnormal
Day 4 ceratobranchial cartilage decreased distance ceratobranchial cartilage, abnormal
Day 4 ceratobranchial cartilage dislocated ceratobranchial cartilage, abnormal
Day 4 ceratobranchial cartilage orientation ceratobranchial cartilage, abnormal
Day 4 ceratohyal cartilage orientation ceratohyal cartilage, abnormal
Day 4 palatoquadrate arch decreased length, abnormal
unc45atr12/tr12; la116Tg; sd2Tg standard conditions Day 4 basibranchial condensed, abnormal
Day 4 ceratobranchial cartilage decreased distance ceratobranchial cartilage, abnormal
Day 4 ceratobranchial cartilage dislocated ceratobranchial cartilage, abnormal
Day 4 ceratobranchial cartilage orientation ceratobranchial cartilage, abnormal
Day 4 ceratohyal cartilage orientation ceratohyal cartilage, abnormal
Day 4 palatoquadrate arch decreased length, abnormal
Day 4 ventral aorta morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 318(2), Anderson, M.J., Pham, V.N., Vogel, A.M., Weinstein, B.M., and Roman, B.L., Loss of unc45a precipitates arteriovenous shunting in the aortic arches, 258-267, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.