FIGURE

Fig. 7

ID

ZDB-FIG-080617-11

Publication

Anderson et al., 2008 - Loss of unc45a precipitates arteriovenous shunting in the aortic arches

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Fig. 7

Rescue and phenocopy experiments confirm the role of unc45a in the kus^tr12 vascular phenotype. Injection of full length (B; n = 90) but not C-terminally truncated (C; n = 13) unc45a mRNA (unc45a^kus, corresponding to the kus^tr12 allele) restored a wild type vascular phenotype to 67% of kus^tr12 mutants (compare to uninjected kus^tr12, A). Conversely, injection of an unc45a translation blocking morpholino (ATG MO, E; n = 144) or exon 13/14 splice donor site blocking morpholino (splice MO, F; n = 60) produced a kus^tr12-like AVM phenotype in 20% and 23%, respectively, of wild type embryos (compare to uninjected wild type, D). A–C, F: 2D confocal projections of 74 hpf TG(flk1:GFP^la116;gata1:dsRed) embryos, labeling endothelial cells green and blood cells red. D, E: 2D confocal projections of angiograms of 74 hpf embryos. Lateral views, anterior to the left. PHS, primary head sinus.

Expression Data

Genes:	DsRed GFP
Fish:	la116Tg; sd2Tg + MO2-unc45a unc45a^tr12/tr12; la116Tg; sd2Tg
Knockdown Reagent:	MO2-unc45a
Anatomical Terms:	aortic arch blood cell blood vessel endothelial cell primary head sinus
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-unc45a la116Tg; sd2Tg + MO2-unc45a unc45a^tr12/tr12; la116Tg; sd2Tg
Knockdown Reagents:	MO1-unc45a MO2-unc45a
Observed In:	angiogenesis blood circulation blood vessel morphogenesis pharyngeal vasculature primary head sinus
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 318(2), Anderson, M.J., Pham, V.N., Vogel, A.M., Weinstein, B.M., and Roman, B.L., Loss of unc45a precipitates arteriovenous shunting in the aortic arches, 258-267, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.