FIGURE

Fig. 2

ID

ZDB-FIG-080617-7

Publication

Anderson et al., 2008 - Loss of unc45a precipitates arteriovenous shunting in the aortic arches

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Fig. 2

Aortic arches 5 and 6 exhibit delayed lumenization and fail to properly connect to the lateral dorsal aortae in kus^tr12 mutants. In wild type embryos, aortic arches 5 and 6 are lumenized by 50 hpf (A) and exhibit strong blood flow by 52 hpf (C). In kus^tr12 (B, D), lumenization is delayed by 2–3 h and blood cells accumulate in distal portions of aortic arch 5 (arrow). This accumulation of blood cells is accompanied by failure of the AA5X to make a patent connection to the LDA [compare E (WT) to F (kus^tr12)]. A–F, 2D confocal projections of TG(flk1:GFP^la116; gata1:dsRed) embryos at 50 hpf (A, B) or 52 hpf (C–F), labeling endothelial and blood cells green and red, respectively. A–D, lateral views, anterior to the left. E, F, dorsal views, anterior to the left.

Expression Data

Genes:	DsRed GFP
Fish:	la116Tg; sd2Tg unc45a^tr12/tr12; la116Tg; sd2Tg
Anatomical Terms:	aortic arch blood cell blood vessel endothelial cell lateral dorsal aorta
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	unc45a^tr12/tr12; la116Tg; sd2Tg
Observed In:	angiogenesis aortic arch 5 aortic arch 6 blood vessel morphogenesis
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 318(2), Anderson, M.J., Pham, V.N., Vogel, A.M., Weinstein, B.M., and Roman, B.L., Loss of unc45a precipitates arteriovenous shunting in the aortic arches, 258-267, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.