ZFIN ID: ZDB-FIG-080604-75
Peri et al., 2008 - Live Imaging of Neuronal Degradation by Microglia Reveals a Role for v0-ATPase a1 in Phagosomal Fusion In Vivo. Cell   133(5):916-927 Full text @ Cell
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 7 Phagolysosomal Fusion Defects in atp6v0a1 Morpholino Knock-Down

(A) Confocal time-lapse analysis of wild-type (A) and atp6v0a1-MO microglia (B) microglia in 3 dpf brains. Time is indicated in minutes and seconds. Microglia are in green marked by GFP expression (Apo-E-GFP) while acidic endocytotic vesicles are marked in red by the LysoTracker. Blue arrowheads mark the formation of phagosomes. Yellow arrowheads mark phagosomal acidification (the vesicle turns red). White asterisks label single acidic vesicles. Fusion between acidic phagosomes is only observed in wild-type.

(C–P) Heterotypic vesicular fusion in wild-type (C–F) and in atp6v0a1-MO microglia (G–P). (E, I, L, and O) NBT-DsRed labeling of phagosomes and phagolysosomes. (F, J, M, and P) DQ-BSA labeling of lysosomes and phagolysosomes. (D, H, K, and N). Merge. Phagosomes are marked by red arrowhead, lysosomes by blue arrowheads and phagolysosomes by purple arrowheads. Phagolysosomes (purple) are only observed in the wild-type (C and D).

(Q) Percentage of the number of phagosomes (red bars), lysosomes (blue bars) and phagolysosomes (purple bars) present in wild-type (n = 15) and morphant cells (n = 15).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zf147Tg + MO1-atp6v0a1a standard conditions Protruding-mouth autophagosome maturation arrested, abnormal
Protruding-mouth phagolysosome assembly arrested, abnormal
zf147Tg; zf148Tg + MO1-atp6v0a1a standard conditions Protruding-mouth autophagosome maturation arrested, abnormal
Protruding-mouth phagolysosome assembly arrested, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Cell, 133(5), Peri, F., and Nüsslein-Volhard, C., Live Imaging of Neuronal Degradation by Microglia Reveals a Role for v0-ATPase a1 in Phagosomal Fusion In Vivo, 916-927, Copyright (2008) with permission from Elsevier. Full text @ Cell