FIGURE
Fig. 5
- ID
- ZDB-FIG-080415-1
- Publication
- Du et al., 2008 - Heat-shock protein 90alpha1 is required for organized myofibril assembly in skeletal muscles of zebrafish embryos
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Fig. 5
Knockdown of Hsp90α1 results in MHC degradation and up-regulation of unc-45b expression. (A) Western blot analysis shows the low levels of MHC proteins in Hsp90α1 knockdown embryos. In contrast, expression levels of actin and myogenin were not affected. (B) RT-PCR analysis of fast-muscle MHC (fMHC), slow-muscle MHC (sMHC), unc-45b, Hsp90α1, and ef-1α expression in control (lanes 1, 3, 5, 7, and 9) or Hsp90α1 ATG-MO injected (lanes 2, 4, 6, 8, and 10) embryos. (C–F) Side views (C and E) or cross-sections (D and F) show unc-45b expression in control (C and D) or Hsp90α1 ATG-MO (E and F) injected embryos at 24 hpf. |
Expression Data
| Genes: | |
|---|---|
| Fish: | |
| Knockdown Reagent: | |
| Anatomical Terms: | |
| Stage: | Prim-5 |
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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Full text @ Proc. Natl. Acad. Sci. USA