FIGURE

Fig. 3

ID

ZDB-FIG-080407-2

Publication

Du et al., 2008 - Heat-shock protein 90alpha1 is required for organized myofibril assembly in skeletal muscles of zebrafish embryos

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Fig. 3

Knockdown of Hsp90α1 expression resulted in myofibril disorganization in skeletal muscles of zebrafish embryos. (A and B) Anti-MHC antibody (F59) staining shows the organization of thick filaments in trunk slow muscles of control-MO (A) or Hsp90α1-ATG-MO (B) injected embryos at 24 hpf. (C and D) Anti-MHC antibody (F59) staining shows the organization of thick filaments in trunk slow muscles of Hsp90α2 ATG-MO (C) or Hsp90α1-E3I3-MO (D) injected embryos at 24 hpf. (E and F) Anti-actin antibody staining shows the organization of thin filaments in control-MO (E) or Hsp90α1-ATG-MO (F) injected embryos at 24 hpf. (G and H) Anti-α-actinin antibody staining shows the organization of the Z-line in control-MO (G) or Hsp90α1-ATG-MO (H) injected embryos at 24 hpf. (I and J) Anti-myomesin antibody staining shows the organization of the M-line in control-MO (I) or Hsp90α1-ATG-MO (J) injected embryos at 72 hpf. (Scale bars: A and B, 25 μm and I, 15 μm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-hsp90aa1.2 WT + MO4-hsp90aa1.1 WT + MO5-hsp90aa1.1
Knockdown Reagents:	MO2-hsp90aa1.2 MO4-hsp90aa1.1 MO5-hsp90aa1.1
Observed In:	myotome M band myotome striated muscle thin filament myotome Z disc sarcomerogenesis skeletal muscle cell myofibril skeletal myofibril assembly slow muscle cell sarcomere
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA