A-E: The avt-expressing cells of the ventral hypothalamus are located at the edges of the shha and titf1a expression domains. Coexpression of avt, titf1a, and shha mRNA was determined by double-label in situ hybridization (ISH). Lateral views, dorsal is up and anterior to the left. The age of the embryos is indicated on each panel in hours postfertilization (hpf). A,C,D,E,G,H: The eyes have been removed. The identity and color of the probes are indicated on each panel. A,C,E,G: Images are merged composites of brightfield and epifluorescence images. The avt-expressing cell groups in the ventral hypothalamus are indicated by black arrows, whereas the avt cell group in the dorsal preoptic area is indicated with a black arrowhead. Scale bars = 100 μm.
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