FIGURE

Fig. 3

ID

ZDB-FIG-080408-1

Publication

Kedde et al., 2007 - RNA-Binding Protein Dnd1 Inhibits MicroRNA Access to Target mRNA

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Fig. 3

Zebrafish Dnd1 (drDnd1) Counteracts Inhibition of Nanos by miR-430
(A) One-cell-stage zebrafish embryos were injected with Dnd1 morpholino or control morpholino. RNA was extracted and subjected to quantitative RT-PCR analysis to compare endogenous levels of nanos1 and vasa to odc, and nanos to vasa.
(B) One-cell-stage zebrafish embryos were coinjected with DsRed-nos1-3′UTR and gfp-vasa-3′UTR together with dead end morpholino or control morpholino.
(C) One-cell-stage zebrafish embryos were coinjected with RNA containing the venus open-reading frame fused to the wild-type nanos1 3′UTR (3′nos1wt), RNA containing the cfp open-reading frame fused to the miR-430-binding site mutated nanos1 3′UTR (cfp-3′nos1mut1) and vasa-dsRed (for labeling the germinal granule for easier identification of germ cells) together with dead end morpholino or control morpholino. Error bars depict the standard error of the mean (SEM); the p value was calculated using t test.

Expression Data

Genes:	ddx4 nanos3 odc1
Fish:	WT WT + MO1-dnd1
Knockdown Reagent:	MO1-dnd1
Anatomical Term:	whole organism
Stage Range:	1-cell to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 131(7), Kedde, M., Strasser, M.J., Boldajipour, B., Oude Vrielink, J.A., Slanchev, K., le Sage, C., Nagel, R., Voorhoeve, P.M., van Duijse, J., Andersson Ørom, U., Lund, A.H., Perrakis, A., Raz, E., and Agami, R., RNA-Binding Protein Dnd1 Inhibits MicroRNA Access to Target mRNA, 1273-1286, Copyright (2007) with permission from Elsevier. Full text @ Cell