Fig. 3

Expression pattern of zebrafish ltk in WT (A?D,F,H,J,K,M,O,Q,S,U,W?Y,AC) and shd^ty82 homozygous embryos (G,I,L,N,P,R,T,V,Z,AD) throughout embryonic development.
Stages indicated in hpf. A?C) ltk-expressing cells in vicinity of eye (lower arrows in A,B) and in premigratory trunk NC (upper arrow in B and C) and in notochord (n). D) Dorsal view of posterior trunk of WT embryo to show ltk expression in scattered cells in dorsolaterally-positioned subset of premigratory NCCs (arrows). E,J) WT embryo treated with phenylthiourea, illuminated with incident light to show iridophore pattern (E), then fixed and processed for ltk ISH (J); individual cells are numbered. F,G,K,L,Q,R) Dorsoventral spread of ltk-expression in WT eye (F,K,Q); cells remain dorsal to eye in shd^ty82 mutants (G,L,R). H,I,M,N,S,T,Y,Z,AC,AD) Cells in premigratory (arrow) and migratory (*) positions and in nascent dorsal and ventral stripes are prominent in WT (H,M,S,Y,AC), but almost absent from shd^ty82 mutants (I,N,T,Z,AD). O,P,U,V,AA,AB,AE,AF) ltk expression pattern closely resembles ednrb1 expression in WT iridophores (AA,AE), but both markers are absent in shd^ty82 homozygous embryos (P,V,AB,AF). W) Plastic section through eye. X) Transverse section of posterior trunk.