PUBLICATION
Leukocyte tyrosine kinase functions in pigment cell development
- Authors
- Lopes, S.S., Yang, X., Müller, J., Carney, T.J., McAdow, A.R., Rauch, G.J., Jacoby, A.S., Hurst, L.D., Delfino-Machín, M., Haffter, P., Geisler, R., Johnson, S.L., Ward, A., and Kelsh, R.N.
- ID
- ZDB-PUB-080331-9
- Date
- 2008
- Source
- PLoS Genetics 4(3): e1000026 (Journal)
- Registered Authors
- Carney, Tom, Geisler, Robert, Jacoby, Arie, Johnson, Stephen L., Kelsh, Robert, Lopes, Susana, McAdow, Ryan, Rauch, Gerd-Jörg, Yang, Xueyan
- Keywords
- Embryos, Zebrafish, Multiple alignment calculation, Phenotypes, Multipotency, Sequence alignment, Pigments, Neural crest
- MeSH Terms
-
- Alleles
- Animals
- Apoptosis/genetics
- Carrier Proteins/genetics
- Carrier Proteins/metabolism
- Chromosome Mapping
- Embryonic Stem Cells/cytology
- Embryonic Stem Cells/enzymology
- Gene Expression Regulation, Developmental
- High Mobility Group Proteins/genetics
- High Mobility Group Proteins/metabolism
- Leukocytes/enzymology
- Melanocytes/cytology
- Melanocytes/enzymology
- Models, Biological
- Multipotent Stem Cells/cytology
- Multipotent Stem Cells/enzymology
- Mutation
- Neural Crest/cytology
- Neural Crest/embryology
- Neural Crest/enzymology
- Phylogeny
- Protein-Tyrosine Kinases/genetics
- Protein-Tyrosine Kinases/metabolism*
- SOXE Transcription Factors
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 18369445 Full text @ PLoS Genet.
Citation
Lopes, S.S., Yang, X., Müller, J., Carney, T.J., McAdow, A.R., Rauch, G.J., Jacoby, A.S., Hurst, L.D., Delfino-Machín, M., Haffter, P., Geisler, R., Johnson, S.L., Ward, A., and Kelsh, R.N. (2008) Leukocyte tyrosine kinase functions in pigment cell development. PLoS Genetics. 4(3):e1000026.
Abstract
A fundamental problem in developmental biology concerns how multipotent precursors choose specific fates. Neural crest cells (NCCs) are multipotent, yet the mechanisms driving specific fate choices remain incompletely understood. Sox10 is required for specification of neural cells and melanocytes from NCCs. Like sox10 mutants, zebrafish shady mutants lack iridophores; we have proposed that sox10 and shady are required for iridophore specification from NCCs. We show using diverse approaches that shady encodes zebrafish leukocyte tyrosine kinase (Ltk). Cell transplantation studies show that Ltk acts cell-autonomously within the iridophore lineage. Consistent with this, ltk is expressed in a subset of NCCs, before becoming restricted to the iridophore lineage. Marker analysis revene kinase (Ltk). Cell transplantation studies show that Ltk acts cell-autonomously within the iridophore lineage. Consistent with this, ltk is als a primary defect in iridophore specification in ltk mutants. We saw no evidence for a fate-shift of neural crest cells into other pigment cell fates and some NCCs were subsequently lost by apoptosis. These features are also characteristic of the neural crest cell phenotype in sox10 mutants, leading us to examine iridophores in sox10 mutants. As expected, sox10 mutants largely lacked iridophore markers at late stages. In addition, sox10 mutants unexpectedly showed more ltk-expressing cells than wild-type siblings. These cells remained in a premigratory position and expressed sox10 but not the earliest neural crest markers and may represent multipotent, but partially-restricted, progenitors. In summary, we have discovered a novel signalling pathway in NCC development and demonstrate fate specification of iridophores as the first identified role for Ltk.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping