ZFIN ID: ZDB-FIG-080325-26
Hendricks et al., 2008 - Disruption of Esrom and Ryk identifies the roof plate boundary as an intermediate target for commissure formation. Molecular and cellular neurosciences   37(2):271-283 Full text @ Mol. Cell Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 7 wnt4a and dnRyk affect commissure formation. Lateral (A) and dorsal (B) view of a 48 hpf embryo, showing expression of wnt4a in the medial habenula. (C) A 3 dpf embryo after injection with a morpholino (MO1) to wnt4a. Habenular afferents have entered the habenula and reached the medial edge (arrows) but have not crossed the roof plate. Axons have innervated the habenula neuropil (arrowhead), which normally occurs only after the commissure has formed. (D) An embryo at 3 dpf after unilateral electroporation with HuC:Gal4/UAS:dnRyk-EGFP at 1 dpf. Abnormal loops and branches are seen within the commissure (n = 15). (E, F) Time series of individual axons show that growth cones reach the contralateral boundary, then bifurcate (arrowheads), turn around and recross the midline (see Supplementary Movies 5 and 6). Time series in (E) was taken without a heated stage, causing slower growth. Time is h:min.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
wnt4 WT standard conditions Long-pec habenula ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-wnt4a standard conditions Long-pec habenular commissure aplastic, abnormal
WT + MO3-wnt4a standard conditions Long-pec habenular commissure aplastic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Molecular and cellular neurosciences for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Molecular and cellular neurosciences, 37(2), Hendricks, M., Mathuru, A.S., Wang, H., Silander, O., Kee, M.Z., and Jesuthasan, S., Disruption of Esrom and Ryk identifies the roof plate boundary as an intermediate target for commissure formation, 271-283, Copyright (2008) with permission from Elsevier. Full text @ Mol. Cell Neurosci.