FIGURE

Fig. S1

ID

ZDB-FIG-080325-113

Publication

Lachnit et al., 2008 - Alterations of the cytoskeleton in all three embryonic lineages contribute to the epiboly defect of Pou5f1/Oct4 deficient MZspg zebrafish embryos

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Fig. S1

Comparison of the egg size of WT and MZspg^m793 embryos. Embryos were obtained by in vitro fertilization. (A, B) Unfertilized oocytes 2 min after activation in egg water and (D, E) fertilized embryos at 1000 cell stage. (A, D) WT and (B, E) MZspg^m793. The image area of the activated oocytes or the yolk cell area was traced and measured using ImageJ software (C, F). Oocytes and embryos at 1 k stage showing no significant difference in size when WT and MZspg^m793 embryos are compared. (C) oocytes: WT = 0.38 ± 0.02 mm²; (E) MZspg^m793 = 0.37 ± 0.02 mm². (F) Measurement of the yolk cell area at 1 k stage. WT = 0.30 ± 0.01 mm²; MZspg^m793 = 0.32 ± 0.01 mm². Scale bar in A: 1 mm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pou5f3^m793/m793 (AB)
Observed In:	whole organism
Stage:	1k-cell

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 315(1), Lachnit, M., Kur, E., and Driever, W., Alterations of the cytoskeleton in all three embryonic lineages contribute to the epiboly defect of Pou5f1/Oct4 deficient MZspg zebrafish embryos, 1-17, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.