Fig. 5

Enhanced lamellipodia formation of EVL cells. Animal pole confocal z-projections of Alexa488-Phalloidin stained embryos during epiboly. (A–F) EVL cells. (A, C, E) WT EVL cells with few detectable lamellipodia. (B, D, F) MZspg^m793 EVL cells with enhanced lamellipodia formation at the cell–cell contact site of EVL cells, projecting toward the deep cells, (A, B) at 60% epiboly, (C, D) at 75% epiboly, and (E, F) at 90% epiboly. Images show z-projections of confocal stacks of images using the depth coding method, to visualize the localization of lamellipodia. Depth scale bar is depicted beneath panels E and F. Scale bar: 20 μm. (G) Measurements of the lamellipodia area including SE: 60% epiboly: WT = 100 ± 0%, MZspg^m793 = 100.75 ± 0% (no lamellipodia detectable). 75% epiboly: WT = 100 ± 40%; MZspg^m793 = 1072 ± 67% (p = 3.157 · 10^- 08). 90% epiboly: WT = 100 ± 20%; MZspg^m793 = 2804 ± 290% (p = 2.563 · 10^- 06). Measurements were performed with Volocity software. Data were normalized to WT results which was set as 100%. Asterisks highlight significant differences. Number of analyzed embryos is depicted within or above bars.