FIGURE

Fig. 1

ID

ZDB-FIG-080323-8

Publication

Li et al., 2007 - Localized rbp4 expression in the yolk syncytial layer plays a role in yolk cell extension and early liver development

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Fig. 1

Expression of rbp4 mRNA during early zebrafish embryogenesis. (A) RT-PCR analysis of rbp4 mRNA in wild-type embryos from 1 hpf to 14 hpf. β-actin was used as loading control. M, 100 bp DNA marker. (B, C) Ventral (B) and lateral (C) view of 12 hpf embryos with rbp4 expression as detected by WISH. (D) Ventral view of 16 hpf embryos with expression of ctsL (red) and rbp4 (blue) as detected by two-colour WISH. (E) Cross section of the two color hybridized embyos in (D) as indicated by the dashed line. (F, F′) Magnified view of boxed region F in Panel (E). F, bright field. F′, compound image of DIC/fluorescence reveals rbp4 expression and position of nuclei detected by DAPI staining. Arrows indicate YSL nuclei. (G), Magnified view of boxed region G in Panel (E).

Expression Data

Genes:	ctslb rbp4
Fish:	WT
Anatomical Terms:	hatching gland whole organism yolk yolk syncytial layer
Stage Range:	4-cell to 14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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