Effects of Rbp4 splicing MO on morphology of embryos. Rbp4 Spl-MO was injected into the blastoderm margin area at 4 hpf with Fluorescein and injected embryos were photographed at 24 hpf and 48 hpf. (A, D) Control embryos at 24 hpf and 48 hpf. (B, E) Fluorescent lateral view image of type I phenotype (without yolk cell extension) morphants at 24 hpf (B) and 48 hpf (E). (C, F) fluorescent lateral view image of type II phenotype (short yolk cell extension) morphants at 24 hpf (C) and 48 hpf (F). White double arrows indicate the length and width of yolk extension. (G) Scheme of splicing morpholino and RT-PCR primer positions in the rbp4 gene. Exons are represented by blue boxes with numbers and introns by white boxes. A red bar indicates the region targeted by Rbp4 Spl-MO. (H) RT-PCR analysis of RNAs from control (Ctrl) (non-injection)/splcing morphlino injected embryos (Spl-MO) and control (Ctrl) (non-injection)/mismatch morpholino injected embryos (Mis-MO) at 24 hpf using the rbp4 primers as indicated in Panel (G) or EF1a control primers.
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