Small heat shock protein (sHSP) expression at 48 hours postfertilization (hpf). A-N: Normal expression (A,C,E,G,I,K,M) and expression after a 1-hr heat shock at 37°C (B,D,F,H,J,L,N). hspb1 is expressed in the mid-hindbrain boundary, lens, heart, and muscles of the head and pharyngeal arches. A: It is also expressed at low levels in rostral somites in the vicinity of the horizontal myoseptum, in the trunk, and caudal spinal cord. B: It is ubiquitously upregulated by heat shock. C,D: hspb4 is expressed in lens (C) and is upregulated by heat shock (D). E,F: hspb7 is expressed in heart both before (E) and after (F) heat shock. G,H: hspb8 is expressed at low levels in somites and muscles of the head and pharyngeal arches (G) and is up-regulated widely by heat shock (H). I,J: hspb9 is expressed at low levels in somites (I) and is upregulated in somites and the dorsal mid- and hindbrains by heat shock (J). K,L: hspb11 is expressed in a few rostral somites (K) and is upregulated in heart, dorsal mid- and hindbrains, and somites (L). M,N: hspb12 is expressed in heart both before (M) and after (N) heat shock. ep, epiphysis; fb, forebrain; h, heart; hb, hindbrain; hyp, hypaxial muscle; le, lens; mb, midbrain; mhb, mid-hindbrain boundary; pa, pharyngeal arch; sc, spinal cord; som, somites. Scale bar = 200μm.
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