ZFIN ID: ZDB-FIG-071129-3
Jazwinska et al., 2007 - Activin-betaA Signaling Is Required for Zebrafish Fin Regeneration. Current biology : CB   17(16):1390-1395 Full text @ Curr. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Adult

Fig. S1 actβA Is Markedly Increased during Fin Regeneration in Comparison to Other Candidate Genes (A) Quantification of expression levels of alk4 and Alk4/5/7 ligands normalized to ubiquitously expressed β-actin1 and β-actin2 reference genes in amputated fins at 0 hpa (n = 3), 6 hpa (n = 4), and 24 hpa (n = 4). The normalized gene expression (NGE) values were calculated with the Pfaffl method and multiplied by a factor of 10,000. squint, cyclops, southpaw, TGFβ2, GDF8, and GDF11 have very low NGE values, indicating that they are not significantly expressed in fins. Error bars indicate the SEM, and n refers to the number of biological samples; each sample was prepared from 15–20 fins. (B) n-fold change in NGE in regenerating fins at 6 hpa and 24 hpa (test samples) relative to cut fins at 0 hpa, which as a calibrator sample were normalized to 1.00. alk4 displays nearly 2-fold induction in fins at 6 and 24 hpa; actβA is the only Alk4/5/7 ligand significantly upregulated during fin regeneration. Error bars indicate the SEM. (C–E) In situ hybridization of fins at 24 hpa. Each panel shows a fragment of a whole-mount (left) and a longitudinal section through the ray (right). In this and subsequent figures, the fins are oriented with the distal edge up. alk4 mRNA (purple) is weakly ubiquitously expressed with upregulation in the blastema (arrows) and the disorganized mesenchyme; "b" indicates bones, "e" indicates epidermis, "m" indicates mesenchyme, "we" indicates wound epidermis, and "bl" indicates blastema (C). actβB mRNA is detected in the entire epidermis (D). No labeling was detected with a squ mRNA antisense probe (E).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
acvr1ba WT standard conditions Adult fin RTPCR
WT physical alteration: anatomical structure Adult blastema ISH
Adult regenerating fin RTPCR
inhbaa WT standard conditions Adult fin RTPCR
WT physical alteration: anatomical structure Adult regenerating fin RTPCR
Adult regeneration epithelium ISH
inhbb WT standard conditions Adult fin RTPCR
WT physical alteration: anatomical structure Adult regenerating fin RTPCR
ndr1 WT physical alteration: anatomical structure Adult Not Detected regenerating fin ISH
tgfb1a WT standard conditions Adult fin RTPCR
WT physical alteration: anatomical structure Adult regenerating fin RTPCR
tgfb3 WT standard conditions Adult fin RTPCR
WT physical alteration: anatomical structure Adult regenerating fin RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.