ZFIN ID: ZDB-FIG-071019-28
Amack et al., 2007 - Two T-box genes play independent and cooperative roles to regulate morphogenesis of ciliated Kupffer's vesicle in zebrafish. Developmental Biology   310(2):196-210 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: 75%-epiboly
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 5-9 somites to Prim-5

Fig. 8 Differentiated KV cells are absent in ntl;tbx16 double mutant and morphant embryos. (A–H) At 24 hpf, ntl mutants (B), tbx16 mutants (C) and ntl;tbx16 double mutants (D) resembled embryos injected with ntl MO (F), tbx16 MO (G) and ntl + tbx16 MO (H), respectively. (I–N) aPKC/aTubulin immunostaining of KV cells at 6–8 SS. Differentiated KV cells (arrowheads in panels I–K, M) were present in wild-type (I, M), ntl mutant (J) and tbx16 mutant (K) embryos and showed characteristic morphologies (see Fig. 2). In contrast, aPKC/aTubulin positive cells were not detected in ntl;tbx16 double mutants (L) or ntl + tbx16 MO embryos (N). (O–P) sox17 RNA expression was present in DFCs (arrow in panels O and P) in uninjected controls (O) and ntl + tbx16 morphants (P) at 70–80% epiboly stages. n = notochord.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
sox17 AB standard conditions 75%-epiboly forerunner cell group ISH
AB + MO1-tbxta + MO3-tbx16 standard conditions 75%-epiboly forerunner cell group ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-tbxta standard conditions Prim-5 post-vent region shape, abnormal
Prim-5 trunk morphology, abnormal
AB + MO1-tbxta + MO3-tbx16 standard conditions 5-9 somites Kupffer's vesicle ciliated cell absent, abnormal
Prim-5 post-vent region shape, abnormal
Prim-5 trunk morphology, abnormal
AB + MO3-tbx16 standard conditions Prim-5 post-vent region shape, abnormal
Prim-5 trunk morphology, abnormal
tbx16b104/b104 (AB) standard conditions 5-9 somites Kupffer's vesicle ciliated cell present, normal
Prim-5 post-vent region shape, abnormal
Prim-5 trunk morphology, abnormal
tbx16b104/b104; tbxtab160/b160 (AB) standard conditions 5-9 somites Kupffer's vesicle ciliated cell absent, abnormal
Prim-5 post-vent region shape, abnormal
Prim-5 trunk morphology, abnormal
tbxtab160/b160 (AB) standard conditions 5-9 somites Kupffer's vesicle ciliated cell present, normal
Prim-5 post-vent region shape, abnormal
Prim-5 trunk morphology, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 310(2), Amack, J.D., Wang, X., and Yost, H.J., Two T-box genes play independent and cooperative roles to regulate morphogenesis of ciliated Kupffer's vesicle in zebrafish, 196-210, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.