FIGURE

Fig. 2

ID

ZDB-FIG-071019-22

Publication

Amack et al., 2007 - Two T-box genes play independent and cooperative roles to regulate morphogenesis of ciliated Kupffer's vesicle in zebrafish

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Fig. 2

KV morphological defects are different in ntl and tbx16 mutants. (A–L) Confocal microscopy of double fluorescent immunostaining of aPKC (red) and aTubulin (green) in KV cells at 8 SS. In wild-type embryos (A–C), aPKC marked the apical surface of Kupffer's vesicle (KV) cells that line the spherical KV lumen and aTubulin labeled cilia protruding into the luminal space. aPKC⁺/aTubulin⁺ KV cells were present in both ntl (D–F) and tbx16 (G–L) mutant embryos. ntl mutants consistently lacked a KV lumen (D–F). In contrast, a KV lumen formed in the majority of tbx16 mutant embryos, but was reduced in size, dysmorphic (G–L) and often accompanied by ‘mini-vesicles’ (arrow in panel L). All confocal micrographs are at the same scale (see scale bar in panel C).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tbx16^b104/b104 (AB) tbxta^b160/b160 (AB)
Observed In:	Kupffer's vesicle Kupffer's vesicle ciliated cell
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 310(2), Amack, J.D., Wang, X., and Yost, H.J., Two T-box genes play independent and cooperative roles to regulate morphogenesis of ciliated Kupffer's vesicle in zebrafish, 196-210, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.